Field
Value
Language
dc.contributor.author
Rossi, Giulia
datacite.creator.affiliationIdentifier
https://ror.org/02fa3aq29;https://ror.org/03dbr7087
en_US
datacite.creator.affiliation
McMaster University;University of Toronto
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-4812-8869
en_US
dc.date.accessioned
2024-03-26T19:20:34Z
dc.date.available
2024-03-26T19:20:34Z
dc.date.issued
2024-03-26
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/bc8c77b4-dcd5-465f-9310-f624aed6f97c
dc.identifier.uri
https://doi.org/10.20383/103.0919
dc.description
Bumble bee populations across the globe are experiencing substantial declines due to climate change, with major consequences for pollination services in both natural and agricultural settings. Using an economically-important species, Bombus impatiens, we explored the physiological mechanisms that may cause susceptibility to extreme heat events. We tested the hypothesis that heat exposure limits the activity of the pentose phosphate pathway (PPP) – a parallel pathway to glycolysis that can use nectar sugar to generate antioxidant potential and combat oxidative stress. Using isotopically-labeled glucose, we tracked PPP activity in B. impatiens at rest, during exercise, and during a post-exercise recovery period under two different temperature regimes (22ºC and 32ºC). We found that the PPP is routinely used by B. impatiens at moderate temperatures, but that its activity is markedly reduced when ATP demands are high, such as during periods of exercise and heat exposure. We also exposed B. impatiens to either 22ºC or 32ºC for 5 hours and assessed levels of oxidative damage (lipid peroxidation, protein carbonyls) and antioxidant potential (reduced (GSH) and oxidized (GSSG) glutathione concentrations). Interestingly, bees exhibited little oxidative damage after the thermal exposure, but we found a lower GSH:GSSG ratio in 32ºC-exposed bees, reflecting lower antioxidant potential. Overall, our study demonstrates that acute heat stress severely limits PPP activity and may constrain antioxidant potential in B. impatiens. The repeated attenuation of this pathway in a warming climate may have more severe physiological consequences for this species, with potential implications for pollination services across North America.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
glucose
en_US
dc.subject
oxidative stress
en_US
dc.subject
climate change
en_US
dc.subject
thermal stress
en_US
dc.subject
pollinators
en_US
dc.subject
metabolic pathways
en_US
dc.title
Heat exposure limits pentose phosphate pathway activity in bumble bees
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/1/published/publication_914/
datacite.publicationyear
2024
datacite.contributor.DataCollector
Giulia Rossi
datacite.contributor.Supervisor
Ken Welch
datacite.date.Collected
2022-06-01/2023-07-31
datacite.resourcetype
Dataset
en_US
datacite.geolocation.geolocationPlace
Southern Ontario;-;Ontario;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060806
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Animal physiology
en_US
frdr.crdc.field_en
Animal physiology, environmental stress
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Physiologie animale
fr_CA
frdr.crdc.field_fr
Physiologie animale, stress environnemental
fr_CA