Description:	Sodium and potassium channels that regulate axonal spike propagation are highly organized at nodes of Ranvier by a spectrin-actin membrane periodic skeleton. STORM-TIRF microscopy was used to define the spatial organization over the soma of a complex of Cav1.3 calcium, RyR2, and IK potassium channels (CaRyK) that generate a slow AHP in hippocampal neurons. Nearest neighbor distance and non-negative matrix factorization analyses identified two spatial patterns as linear rows of 3-8 immunolabeled clusters with 155 nm periodicity that extended to branchpoints, or as isolated clusters with 600-800 nm separation. The same patterns were detected for spectrin βII and the actin linking proteins actinin I and II that substantially overlapped with CaRyK complex clusters. The data reveal a close correspondence between CaRyK complex proteins and a net-like structure of spectrin βII across the soma that coordinates the spatial organization of channels that control the pattern of spike output.
Notes:	Item exited embargo and became publicly available on 2024-04-24
Authors:	Sahu, Giriraj; Hotchkiss Brain Institute, Cummings School of Medicine, University of Calgary; University of Calgary; 0000-0001-6566-9563 Greening, Dylan; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; Department of Clinical Neuroscience, Cumming School of Medicine, University of Calgary; University of Calgary; 0009-0006-1756-571X Wilten, Nicola; Department of Cell Biology & Anatomy, Cumming School of Medicine, University of Calgary; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; University of Calgary Turner, Raymond W; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; Department of Clinical Neuroscience, Cumming School of Medicine, University of Calgary; University of Calgary
Keywords:	CaV1.3 KCa3.1 RyR2 spectrin cytoskeleton ER-PM junction slow AHP hippocampus
Field of Research:	Basic medicine and life sciences > Neurosciences, medical and physiological and health aspects > Neurosciences, medical and health and physiological aspects, not elsewhere classified
Publication Date:	2024-04-24
Publisher:	Federated Research Data Repository / dépôt fédéré de données de recherche
Funder:	Canadian institute of health research; The molecular choreography of ion channel expression (RWT); Ref# PJT-169007 Natural Sciences and Engineering Research Council of Canada; Discovery grant
URI:	https://doi.org/10.20383/103.0809
Geographic Coverage:	City Calgary Province / Province / Territory Alberta Territory   Country Canada