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Multimodal data with wide-field GCaMP imaging and sub-cortical/cortical single unit recording (2015)
| Description: | This dataset includes raw wide-field GCaMP image sequences and time stamps of spike trains simultaneously recorded with GCaMP imaging and processed GCaMP images and spike trains: df/f0 with 0.1-10 band pass filter and spike train binned in one frame time. Most experiments were performed toward the end of the mouse light cycle. Mice were anesthetized with isoflurane (0.5–1.0%) or urethane during data collection. Images of the cortical surface were recorded through a pair of front-to-front video lenses coupled to a CCD camera. Calcium indicators were excited with blue-light-emitting diodes (470 nm) with bandpass filters (467–499 nm). Emission fluorescence was filtered using a 510–550 nm bandpass filter. For in vivo single unit recording, the electrode was inserted into the right hemisphere with a 45 degree angle from the lateral surface of the cortex to avoid contact with the region where wide-field imaging was performed. The electrode was first held in the cortex for cortical recordings and then advanced into the sub-cortical areas. |
| Authors: | Xiao, Dongsheng; University of British Columbia |
| Keywords: | wide-field GCaMP imaging sub-cortical/cortical cellular electrophysiology in vivo optogenetics |
| Field of Research: | Clinical medicine > Clinical sciences > Neurology
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| Publication Date: | 2019-07-30 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| URI: | https://doi.org/10.20383/101.0170 |
| Related Identifiers: |
This dataset is a supplement to
This dataset is continued by
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| Appears in Collections: | UBC Brain Circuits |
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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Xiao, D. (2019). Multimodal data with wide-field GCaMP imaging and sub-cortical/cortical single unit recording (2015). Federated Research Data Repository. https://doi.org/10.20383/101.0170
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