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Characterization of high mountain glacier microbiota following inundation with wildfire soot
| Description: | Glacier ecosystems are shrinking at an accelerating rate due to changes in climate, but also due to increased deposition of soot, which has resulted in darkening of glacier surfaces and subsequent changes in absorption of light, associated heat, and altered microbial communities. In this study, compositions of microbial communities on surfaces of the Athabasca Glacier (Alberta, Canada) were measured by use of DNA metabarcoding. Three matrices, glacier ice, cryoconite hole, and glacial surface sediment, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata. |
| Notes: | Item exited embargo and became publicly available on 2022-01-01 |
| Authors: | Esser, Milena; University of Saskatchewan;  0000-0002-2802-6160Ankley, Phillip; University of Saskatchewan; 0000-0002-5883-6148Aubry-Wake, Caroline; University of Saskatchewan; 0000-0002-9452-8580Xie, Yuwei; University of Saskatchewan; 0000-0001-5652-6413Baulch, Helen; University of Saskatchewan; 0000-0001-9018-4998Hoggarth, Cameron; University of Saskatchewan Hecker, Markus; University of Saskatchewan; 0000-0002-7237-6192Hollert, Henner; Goethe University Frankfurt; 0000-0001-5776-5619Giesy, John; University of Saskatchewan; Baylor University; 0000-0003-1931-9962Pomeroy, John; University of Saskatchewan; 0000-0002-4782-7457Brinkmann, Markus; University of Saskatchewan; 0000-0002-4985-263X |
| Keywords: | Athabasca Glacier ecosystem microbiome soot deposition PAHs |
| Field of Research: | Biological sciences > Microbiology > Microbiome
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| Publication Date: | 2022-01-01 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Canada First Research Excellence Fund (CFREF); Global Water Futures Program (GWF) Global Water Futures (GWF); Next generation solutions to ensure healthy water resources for future generations |
| URI: | https://doi.org/10.20383/102.0528 |
| Geographic Coverage: | Place Name Athabasca Glacier Province / Province / Territory Alberta Territory Country Canada |
| Geographic Point: | Latitude 52.19182 Longitude -117.25165 |
| Appears in Collections: | Global Water Futures |
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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Esser, M., Ankley, P., Aubry-Wake, C., Xie, Y., Baulch, H., Hoggarth, C., Hecker, M., Hollert, H., Giesy, J., Pomeroy, J., Brinkmann, M. (2022). Characterization of high mountain glacier microbiota following inundation with wildfire soot. Federated Research Data Repository. https://doi.org/10.20383/102.0528
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