Characterization of high mountain glacier microbiota following inundation with wildfire soot
| Description: | Glacier ecosystems are shrinking at an accelerating rate due to changes in climate, but also due to increased deposition of soot, which has resulted in darkening of glacier surfaces and subsequent changes in absorption of light, associated heat, and altered microbial communities. In this study, compositions of microbial communities on surfaces of the Athabasca Glacier (Alberta, Canada) were measured by use of DNA metabarcoding. Three matrices, glacier ice, cryoconite hole, and glacial surface sediment, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata. |
| Notes: | Item exited embargo and became publicly available on 2022-01-01 L’élément est sorti de sa période d’embargo et est devenu public le 2022-01-01 |
| Authors: | Esser, Milena; University of Saskatchewan; https://orcid.org/0000-0002-2802-6160  Ankley, Phillip; University of Saskatchewan; https://orcid.org/0000-0002-5883-6148 Aubry-Wake, Caroline; University of Saskatchewan; https://orcid.org/ 0000-0002-9452-8580 Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 Baulch, Helen; University of Saskatchewan; https://orcid.org/ 0000-0001-9018-4998 Hoggarth, Cameron; University of Saskatchewan Hecker, Markus; University of Saskatchewan; https://orcid.org/0000-0002-7237-6192 Hollert, Henner; Goethe University Frankfurt; https://orcid.org/ 0000-0001-5776-5619 Giesy, John; University of Saskatchewan; Baylor University; https://orcid.org/0000-0003-1931-9962 Pomeroy, John; University of Saskatchewan; https://orcid.org/0000-0002-4782-7457 Brinkmann, Markus; University of Saskatchewan; https://orcid.org/ 0000-0002-4985-263X |
| Keywords: | Athabasca Glacier ecosystem microbiome soot deposition PAHs |
| Field of Research: | Biological sciences > Microbiology > Microbiome
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| Date: | 1-Jan-2022 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| URI: | https://doi.org/10.20383/102.0528 |
| Geographic Coverage: | Place Name Athabasca Glacier Province / Province / Territory Alberta Territory Country Canada |
| Geographic Point: | Latitude 52.19182 Longitude -117.25165 |
| Appears in Collections: | Global Water Futures |
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Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Esser, M. , Ankley, P. , Aubry-Wake, C. , Xie, Y. , Baulch, H. , Hoggarth, C. , Hecker, M. , Hollert, H. , Giesy, J. , Pomeroy, J. , Brinkmann, M. (2022) Characterization of high mountain glacier microbiota following inundation with wildfire soot. Federated Research Data Repository. https://doi.org/10.20383/102.0528
